Hi Debajyoti, For my low pH phasing (pH 3) the thing that worked for me was the "Magic Triangle." It works best at Cu K alpha (i.e. home source). There is a "Mad Triangle" that works at synchrotron sources as well. You can get it all made up from Hampton or Jena Biosciences but you can also buy the chemical direct from Fisher or Sigma and make it up in LiOH or KOH for super cheap. You can check out the papers here: http://www.ncbi.nlm.nih.gov/pubmed/19020356 or here: http://www.ncbi.nlm.nih.gov/pubmed/19851024
Best of luck, Katherine On Wed, Nov 17, 2010 at 4:31 AM, Debajyoti Dutta < debajyoti_dutt...@rediffmail.com> wrote: > > Hi All, > > Sorry for a non CCP4 question. I have been trying to phase a protein > structure using different heavy atom derivatives. The problem is the > crystallization pH is very low (from 2.8 to 3.5). I will be highly benefited > if anybody kindly suggests me the possible heavy atom salts to try > > sincerely > Debajyoti > > > <http://sigads.rediff.com/RealMedia/ads/click_nx.ads/www.rediffmail.com/signatureline....@middle?>
