To make it clearer, I'm looking for successful examples of crystallization of phosphorylated proteins, especially ones containing multiple phosphorylation sites, and would like to access crystallizability of these protein types. I want to produce and crystallize protein at its phosphorylation state but I learned phosphorylation may cause inhomogeneity problem and interfere with crystallization. It's not a good option to replace these sites anyway because there are many and may affect native structure of the protein.
Any input would be appreciated, Sally. On Wed, Nov 10, 2010 at 1:26 PM, Atlanta Cook <c...@biochem.mpg.de> wrote: > Perhaps you might pose a more directed question... Do you want to produce > phosphorylated protein? Are you having trouble crystallising a protein that > is phosphorylated? > > > Atlanta > > > > > On 10 Nov 2010, at 11:09, Sally Pham Thanh Van wrote: > >> Dear all, >> >> Could you please tell me any information regarding to crystal >> structures of phosphorylated proteins? Your input would be >> appreciated. >> >> Best regards, >> Sally. > >
