Hello all; I have successfully expressed a membrane protein in Pichia pastoris, however I am having a difficult time with cell lysis. I have used a Avestin emulsiflex to lyse them, however I have had many difficulties with the system clogging up, and parts wearing out with the high pressures.
So I am wondering what other people out there use to lyse their Pichia? In particular we have been considering a microfluidizer a Retsch mixer mill a TS-series cell disruptor (Constans systems) Any thoughts on these options, or other systems would be much appreciated! Best regards, Cory Cory Brooks, Ph.D. Postdoctoral Fellow University of Alberta
