The other thing you can try to do is "the-cheap-men-silver-stain"
scan your gel and bump up the contrast, you'd be surprised what you can detect even from a regular stained gel. Best results are if you make the gel first as a greyscale image. Google for Neuhoff stain and bump up the phosphoric acid to 10%. Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Nov 2, 2010, at 10:19 AM, Jim Pflugrath wrote: > It reads like you need to run a lane or two with a positive control of some > kind. Can you grow lysozyme, glucose isomerase, hemoglobin or other crystals > of a protein around the same expected molecular weight and try run on the gel > lanes with about the same amount of crystalline volume as your putative > protein crystals? > > From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of > xaravich ivan > Sent: Monday, November 01, 2010 9:51 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] Crystal gel band > > Hi everyone, > I have grown some crystals after micro-seeding starting from thin-small > needles from needle-clusters. These crystals are larger in size than the > needles but are comparable to the shape and don't look like salt crystals. > But I cannot see the bands( its a complex) in the SDS-PAGE.I do not have a > home source,handy and would like to send these to the synchrotron. > > Is it possible to NOT see a band of protein crystals in SDS-PAGE, if, say, > the amount of protein is < 1uG? > Has anyone experienced such a thing (no band in gel, but crystal diffracts)? > It would be nice if I get observations/suggestions. > > ivan
