How can you have 3 copies and one strong pseudo translation?You probably
need an even number of molecules with pairs related by the translation
vector.
Eleanor
..
On 10/17/2010 05:48 AM, Mike John wrote:
Hi all,
The data is 2.2A, p41212, unit cell has c axis twice longer
than a or b. Data processed by HKL2000, the diffraction pattern shows
Strong-weak-weak in C direction, indicating translational
pseudo-symmetry.
Xtriage analysis confirmed the pseudo-symmetry (large Patterson off-origin
peak).
The model is 100% identical searching prob.
Phaser MR solved the structure easily with good Z-score and LLG(3 copies/a.u).
The problem comes during the refinement:
The R-free converged around 50% (R-factor 38%) and never go down even though I
tried hard including, twin possibility.
Seeking help to lower the R-free. Thank you very much
Mike
