I used NaF in the precipitant thrice for three different proteins to be
crystallized & each time I got crystals and they were all salt crystals ! I
thought low solubility of NaF causes this quick crystallization of salt.
Recently I got crystals of a protein at 20 degree grown in gradient
concentration of AmSO4 as precipitant.In some cover-slips I got precipitate
initially but I kept the tray at RT for mounting at home-source & after some
time I saw small crystals appeared in those drops which contained precipitate
initially.Though the small crystals never grew enough for mounting. Some drops
were used for seeding,small crystals appeared but they dissolved/disappeared at
RT, again reappeared after several weeks as larger one.
