Hi all,
I have collected a data at 2.9 Å and the solved the structure using phaser .
the space group comes to be P2 21 21. There are 4 molecules in Assymetric unit
and an octamer is generated according to the symmetry. But after repeated
rounds of rigid body refinement with REFMAC5 and model building with coot the
R factor had been struck at 40% and R free at 50%.
So my first question is whether my solution after phaser is correct. And if it
is how can i lower the R factor and Rfree.
The second question is it possible to have such a large difference between R
factor and R free.
Thanks in advance
