Dear experts, Thank you very much for your input. Here are my answers for your questions:
>To me, the first 3 pictures suggest that there is a faint greenish tinge in the little shapes (hopefully >crystalline) there. I guess the idea of the Topaz chip is that you can take it as is to the x-ray beam or am I >wrong? - I didn't see the greenish tinge in the original images. I think the color of the images was modified when I convert to pdf format. - The screening Topaz chip cannot apply for diffraction. They have a different chip for in-situ diffraction but we didn't use that chip in this case. >What GFP did you use, your pH is 4.6 or something around there according to your picture. If the >pH is true GFP will be colorless. You certainly have some more GFP lying around (without your >protein fused to it), just titrate it and see what pH dependency you have. - The protein was fused with GFPuv. It also shows the beautiful green color at the concentration 30mg/ml in the low pH buffer (20mM sodium acetate 4.6, 100mM NaCl, 0.2% Cymal5). Do you think that the crystal getting bigger is a good sign of protein crystal? My best regards, * TriNgo * Structural Biology Lab School of Medicine - Sungkyunkwan University Phone: 031-299-6150
