Dear Daniel, probably the first and easiest thing you could check is whether the freezing conditions are suboptimal, by a room temperature measurement.
If that fails, you can add another purification step to your complex (certainly worth a try), and/ or a restricted proteolysis, which might chop off some floppy loops without disrupting the complex. You might also try to solve the structure at 8A - seems possible if I remember correctly from the ECM... Tim On Thu, Sep 09, 2010 at 04:23:30AM +0900, Daniel Lee wrote: > Hi Cyrstallographers.... > > I made the crystal of triple complex and the M.W is about 500 kDa. > However, the diffraction is about 8 A. > At first, the crystal form was needle. the needle form was changed by > addtive screening. > > the crystal condition is under 1 M ammonium formate with Hepes (pH 7.5). > Crystal size is more than 0.4 um. > > I tried to do annealing, dehydration and glutaraldehyde use but It > does not increase the diffraction. > > I need some help. > > I will very appreciate your all comments. > > Thanks > > Best, > Daniel -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
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