Re: [ccp4bb] non-identical complexes in the asymmetric unit

[Roger Rowlett]

I don't think this is uncommon at all. For example, we published a structure where 10 chains did not bind ligand at all, and 2 chains did in the ASU (see PDB 3E3I). We have also recently solved a structure where two active sites in the ASU are in different states. Cheers. On 9/8/2010 12:50 PM, Rongjin Guan wrote: Dear All,   I have a structure with two complexes in the asymmetric unit, and the interactions on the interface are not the same in the two complexes. Briefly, there are two additional hydrogen bonds in one complex, but not in the other. This coule be due to crystallization artefact, but may have other explanations.   Can anyone direct us to some references where this has been discussed before?   Thank you very much   Rongjin Guan     -- Roger S. Rowlett Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@colgate.edu