Hi Qing, I would recommend either the use of GFP as mentioned by Jacob or a his-tag or a flag-tag. C-terminal tagging is preferred to prevent interference with signal sequences at the N-terminus of the protein. Flag tag is really good for detection , the commercial antibodies for detection are really great, however it is not that great, in my hands, when it comes to purification of a membrane protein (in presence of detergent) I prefer his-tags to flag-tags in this case. You can use a his-flag to combine both advantages.
Hope this helps -- Pascal F. Egea, PhD Assistant Professor UCLA, David Geffen School of Medicine Department of Biological Chemistry 314 Biomedical Sciences Research Building office (310)-983-3515 lab (310)-983-3516 email pe...@mednet.ucla.edu
