Dear Qing,
  Many structures have been solved that way.  Make sure you try solvent 
modification (flattening / flipping) on your map.  This is because SAD will 
give two equally-probably estimates for the phase.  The initial, unmodified map 
will use the average of the two, but solve modification should be able to break 
the amibiguity.
  It sounds like you're new to crystallography - do you have a local advisor to 
get help from?
     Phoebe

=====================================
Phoebe A. Rice
Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
http://www.rsc.org/shop/books/2008/9780854042722.asp


---- Original message ----
>Date: Mon, 30 Aug 2010 20:32:26 +0200
>From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> (on behalf of Jane Bailey 
><jbailey.x...@googlemail.com>)
>Subject: [ccp4bb] About SAD phasing  
>To: CCP4BB@JISCMAIL.AC.UK
>
>   Hi, All
>
>   I would like to ask whether is it possible to
>   allocate the Se site and obtain the phase by just
>   using the SAD data set (no native dataset used)?
>
>   Thanks,
>   Qing

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