I think you need a special trick to express C-type heme proteins.
For B hemes (protoheme, protoporphryn 9), which I think is what the P450's have, E.coli is always expressing several proteins which contain it. But I guess there may be some way to up the production in case of an overexpresser. Hari Namboodiri wrote:
Hi CCPers Can anyone provide insights about expressing heme containing proteins in E.col? Does E.coli need any porphyrin precursor during expression or you need special E.coli strains. I have references mentioning delta-aminolevulinic acid for one ortholog but none for another. The enzyme is CYP51. Thanks Hari
