Nevertheless, what do you have in mother liquor/protein buffer? On Thu, 2010-08-12 at 17:24 +0200, wrote: > Dear All, > > I am refining structure of protein at 1.7A. It is enzyme with 3 > histidine residues in the active site, which are chelating metal (at > the moment I built in calcium but I do not know for sure, which metal > is bound there). I can see additional density "on top" of metal, which > I can not identify: > http://picasaweb.google.com/j.a.wojdyla/Density# > None of the crystallization or protein buffer's components could fit > there and it seems to be something picked up during expression. > I would be very grateful for any suggestions. > > Thank you in advance. > Justyna
-- Edwin Pozharski, PhD, Assistant Professor University of Maryland, Baltimore ---------------------------------------------- When the Way is forgotten duty and justice appear; Then knowledge and wisdom are born along with hypocrisy. When harmonious relationships dissolve then respect and devotion arise; When a nation falls to chaos then loyalty and patriotism are born. ------------------------------ / Lao Tse /
