I want to do cloning of a 40 Kd protein in pRSETA, and pGEX-KT vector. I don't have any idea about protein solubility, its multimeric form, stability and disorder etc. There is nothing known in the literature also. Is there any software that can predict these parameters, so that i can decide which domain i need to clone for soluble and stable protein purification. Vikrant
------------------------------------------------------------------------------------------------------------------------ Junior Research Fellow (CSIR) Lab No. 101, Dr. Varma Lab Cancer Research Institute Advance Centre for Treatment, Research and Eduction in Cancer (ACTREC) Tata Memorial Hospital, Kharghar, Navi Mumbai-410210 #####################################################################