Thurs., July 29th 2010
EBI
Morning,
I think Andrew meant PDB 2gd1 and 1gd1 (has 4 NAD)
DOI 10.1016/0022-2836(88)90130-1
Miri
On Wed, 28 Jul 2010, A Leslie wrote:
Hi Fred,
If your tetramer show negative cooperativity between the 4 sites for
ligand binding, then it is possible to get a tetramer with (say) only one
subunit occupied by ligand, which can introduce considerable asymmetry if
ligand binding gives rise to a hinge type closure of two domains around the
ligand site. An example of this is glyceraldehyde 3-phosphate dehydrogenase
which has been solved with one, two (and possibly 3) NADs bound per tetramer.
I'm afraid you will need to do a literature search to find the references,
but AJ Wonacott will be one of the authors.
Cheers
Andrew
On 28 Jul 2010, at 19:31, Fred wrote:
Dear CCP4bb,
Could someone please, point me to some references about non-symmetric
tetramers? If I have a tetramer composed by 4 identical subunits, it'll
always have a P4 point group symmetry?
Thank in advance,
Tomb
