If you used EMAN on single particles (http://blake.bcm.tmc.edu/eman/)
proc3d yourmap.mrc dummymap.mrc apix=?.?? calcsf=sf.dat
sf.dat gives the F**2/s distribution for the map - with max=0.0
I think so you will need to scale.
Steve.
Quoting Andreas Förster <docandr...@gmail.com>:
Dear all,
the other day I obtained SAXS data from which a low-resolution
structural model was calculated. The model is simpler/less complex
than one of the same protein that we obtained with cryo-EM.
Is there a way to estimate theoretical SAXS data from a cryo-EM
reconstruction to compare with the obtained raw data? Is there a
program that does for a reconstruction what CRYSOL does for pdbs? I
understand that there would be a huge amount of handwaving involved,
but it might help us reconcile our models.
Thanks.
Andreas
--
Andreas Förster, Research Associate
Paul Freemont & Xiaodong Zhang Labs
Department of Biochemistry, Imperial College London
http://www.msf.bio.ic.ac.uk
______________________________________________________________________
S.M. Prince Phone: +44 (0)161 306 8919
Faculty of Life Sciences FAX: +44 (0)161 306 5201
Manchester Interdisciplinary Biocentre
University of Manchester
131 Princess Street e-mail: steve.pri...@manchester.ac.uk
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