Hi Subhangi
phenix.fobs_minus_fobs_map
will compute this map for you.
Usage examples:
phenix.fobs_minus_fobs_map f_obs_1_file=data1.mtz f_obs_2_file=data2.sca
f_obs_1_label=FOBS1 f_obs_2_label=FOBS2 model.pdb
phenix.fobs_minus_fobs_map f_obs_1_file=data.mtz f_obs_2_file=data.mtz
f_obs_1_label=FOBS1 f_obs_2_label=FOBS2 phase_source=model.pdb
high_res=2.0 sigma_cutoff=2
Or (using the GUI): Maps -> Isomorphous difference maps.
Note, both inputs MTZs should have similar crystal symmetry.
Let me know if you have questions.
Pavel.
PS> This is a non-CCP4 tool.
On 7/12/10 1:53 PM, Subhangi Ghosh wrote:
Hi All,
I have two different datasets of the same protein, for example dataset A and
dataset B. I want to make a difference map of [ Fo (of set A) - Fo (of set B)].
(I'll use phases of another solved structure from an isomorphous crystal). For
this I'll have to scale two different mtz files (associated with set A and B
respectively) but keep them separate after scaling. Is there anyway that I can
do this?
Thank you,
Subhangi
