Hi all, I am looking to obtain plasmids encoding the deglycosylating enzymes peptide-N-glycosidase F and endoglycosidase F1 for expression as fusion proteins with GST. They were described in the following paper:
Deglycosylation of proteins for crystallization using recombinant fusion protein glycosidases. F. Grueninger-Leitch, A. D'Arcy, B. D'Arcy, and C. Chène Department of Gene Technologies, Pharma Preclinical Research, F. Hoffmann-La Roche AG, Basel, Switzerland. Protein Sci. 1996 December; 5(12): 2617–2622. Has anyone here had any luck obtaining them? I understand that a materials transfer agreement is needed. I have tried contacting the authors and Hoffmann-La Roche via four difference channels and have met with a wall of silence! Any help here would be appreciated! With thanks, a++
