I've a protein sequence of known domain. Based on structure alignment, I've got a alignment of those with known structures. Then how to add my
sequence to the alignment?Any suggestions?

Hi Yuan,
You can use UCSF Chimera's Multalign Viewer tool to do this. Just open your alignment with that tool and use the "Edit->Add Sequence" menu item to add your sequence to the alignment.

UCSF Chimera: http://www.cgl.ucsf.edu/chimera/
Multalign Viewer tool: 
http://www.cgl.ucsf.edu/chimera/current/docs/ContributedSoftware/multalignviewer/framemav.html
specifically the "Add Sequence" part: 
http://www.cgl.ucsf.edu/chimera/current/docs/ContributedSoftware/multalignviewer/multalignviewer.html#mavmenu-edit

--Eric

                        Eric Pettersen
                        UCSF Computer Graphics Lab
                        http://www.cgl.ucsf.edu


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