You've also applied BRAIN 2.0 ? I mean looked at homologous structures, superimposed them and decided which parts are to be removed ? Never trust programs :-) There could be a flexible alpha helix which if you removed it would have given you in all programs a solution.
it's Monday, Jürgen On May 24, 2010, at 10:24 AM, Paul Lindblom wrote: > The last molrep job just finished and it found only an odd solution. So I > think I will try to get my phases elsewhere. But I am somewhat astonished > that there are still enough cases you can't solve by MR. > > Thanks to all who replied. Here is a list of servers/programs to find a MR > model: > > http://www.ebi.ac.uk/pdbe-srv/view/ > > http://www.ebi.ac.uk/Tools/fasta33/index.html > > http://meta.bioinfo.pl/submit_wizard.pl > > XtalPred > http://ffas.burnham.org/XtalPred-cgi/xtal.pl > > Balbes http://www.ysbl.york.ac.uk/~fei/balbes/ > > use the OCA browser for FASTA searches of the PDB > > Modeller or Rosetta (both also available as web servers) > > ensemble of many proteins with Phaser > > FFAS server maintained by the Godzik lab > > generate some models using the "Phyre" server ( > http://www.sbg.bio.ic.ac.uk/~phyre/ ) and feed the best .pdbs into Mr Bump. > > > > > - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/
