When X-ray crystallographers not so many years ago thought they are the

salt of the earth, of science and then some (well, some believe so

to this date), the same attitude prevailed (data holds). Once every

person with access to Google does it, that secrecy slowly disappears.

 

What also helps is a few major fups, which promotes community

response by those who have nothing to hide. Fups tend to happen at the

transition from expert science to Google science. EM is probably not there
yet.

So, talk to you colleagues, question every structure, and make the 

argument that science without data is non-falsifiable in Popper's sense 

and thus en par with quackery and superstition.

 

That should make you a lot of friends (or at least it will draw a 

response).   

 

Cheers, BR

 

 

From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Filip
Van Petegem
Sent: Tuesday, May 18, 2010 4:48 PM
To: [email protected]
Subject: [ccp4bb] electron microscopy: where open access fails

 

Dear colleagues,

 

whereas data sharing for most crystallographers appears to be a no-brainer,
making coordinates and (most of the time, hopefully) structure factors
available, it seems the electron microscopists are drastically lagging
behind when it comes to making data available.

 

Many cryoEM structures are still being published without the corresponding
maps being deposited in the EM database.  In one particular case, I was
interested in looking at a cryoEM map from a paper published in a
well-renowned open access journal starting.  The paper contains the EMDB
accession codes for the maps, but these maps appear to be 'on hold' since
over a year.  Enquiry with the authors delivered a firm 'no' in releasing
the maps:  they claim it is OK to keep the maps on hold for 2 years, simply
because the EMDB gives the option to do so.  A further enquiry with the
journal editors led to no avail: despite the clear journal policy on sharing
both manuscripts and data, they were also unable to force the authors to
release their maps, now ~13 months after publication of the paper.  The fact
that this was in an open access journal makes this all the more shocking.

 

It is striking to see how much still has to be done to lift the cryoEM world
up to the same standards as the crystallographic community (when it comes to
sharing data, at least). Structures can simply be published without anybody
being able to check the validity, let alone use it for obvious experiments
such as docking crystal structures, integrative protein structure modeling,
etc. 

 

With many structural targets going towards bigger and more challenging,
combining cryoEM data with crystal structures is going to become more and
more important. What can we, crystallographers, do to stimulate data-sharing
in the cryoEM world?

 

(My apologies to the cryoEM people on this bulletin board:  if you have been
making your maps available, you'll agree that clearly not everybody does...)

 

 

Filip Van Petegem

-- 
Filip Van Petegem, PhD
Assistant Professor
The University of British Columbia
Dept. of Biochemistry and Molecular Biology
2350 Health Sciences Mall - Rm 2.356
Vancouver, V6T 1Z3

phone: +1 604 827 4267
email: [email protected]
http://crg.ubc.ca/VanPetegem/

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