Hi Chris, Isopropanol can act as a cryoprotectant. Can't say whether 25% are enough. It dependes also if there are other ingredients that can help to get cryo conditions (e.g. salts, PEGs, glycerol). Maybe you want to check the mother liquor if there will be any ice ring formation after freezing (or check the loop under the microscope/camera of the xray source). Evaporation of isopropanol (comparable to EtOH) during mounting should not be a serious problem.
HTH Jan --- Chris Meier <crystallogra...@christophmeier.com> schrieb am Fr, 9.4.2010: Von: Chris Meier <crystallogra...@christophmeier.com> Betreff: [ccp4bb] freezing crystals grown in isopropanol condition An: CCP4BB@JISCMAIL.AC.UK Datum: Freitag, 9. April, 2010 10:38 Uhr MessageDear all, I have a protein which crystallizes in 25% isopropanol, at pH4.5. Does anyone have experience freezing crystals grown in such a condition? What cryoprotectants should I try? Can isopropanol itself act as a cryoprotectant? Any suggestions on how to deal with isopropanol evaporation during mounting? Many thanks and best wishes, Chris __________________________________________________ Do You Yahoo!? Sie sind Spam leid? Yahoo! Mail verfügt über einen herausragenden Schutz gegen Massenmails. http://mail.yahoo.com
