Hi, Have you also considered substituting the components of your initial crystallization hit. For example, you can substitute the original PEG with others PEGs. You can also systematically replace the cations (CaCl2, MgCl2, etc) and anions (CaCl2, CaOAc, etc) from the original conditions. This approach has worked very well in the past for a number of crystals that were initially very thing needles or very thin plates.
Along this line, CdCl2 is also a nice additive that seems a little underused. See Trakhanov S<http://www.ncbi.nlm.nih.gov/pubmed?term=%22Trakhanov%20S%22%5BAuthor%5D>, Kreimer DI<http://www.ncbi.nlm.nih.gov/pubmed?term=%22Kreimer%20DI%22%5BAuthor%5D>, Parkin S<http://www.ncbi.nlm.nih.gov/pubmed?term=%22Parkin%20S%22%5BAuthor%5D>, Ames GF<http://www.ncbi.nlm.nih.gov/pubmed?term=%22Ames%20GF%22%5BAuthor%5D>, Rupp B <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Rupp%20B%22%5BAuthor%5D>, Protein Sci. <javascript:AL_get(this,%20'jour',%20'Protein%20Sci.');> 1998 Mar;7(3):600-4. Good Luck, Melanie On Wed, Apr 7, 2010 at 10:46 AM, Debajyoti Dutta < debajyoti_dutt...@rediffmail.com> wrote: > > Hi All, > > I am dealing with a protein that crystallizes in 1D. The broom stick > crystals does not yield with any improvement w.r.t their dimension. I have > tried using different concentration of salt, ppt and pH around the parent > condition, even tried seeding and temperature changing. However, any of > these method does not help. > > Any suggestion is welcome. > > Thank you in advance. > > Sincerely > > Debajyoti > > <http://sigads.rediff.com/RealMedia/ads/click_nx.ads/www.rediffmail.com/signatureline....@middle?>
