Dear all, I would like to know which is the more suitable method of using 2,3-Butanediol as cryoprotectant? Addition in to reservoir buffer or crystal soaking or both can be tried?
Cheers Gauri ------ I am sure both could be tried, but have found quite few papers mentioning brief soaking with 2,3-butanediol: Purification, crystallization and X-ray diffraction analysis of the extracellular part of the human Fc receptor for IgA, Fc[alpha]RI (CD89) http://scripts.iucr.org/cgi-bin/paper?cnor=bw5002 5-10 seconds Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus http://scripts.iucr.org/cgi-bin/paper?cnor=gr2202 described as 'briefly' Crystallization and preliminary X-ray analysis of recombinant human acid [beta]-glucocerebrosidase, a treatment for Gaucher's disease http://scripts.iucr.org/cgi-bin/paper?cnor=pu0086 described as 'briefly' Crystallization and preliminary X-ray crystallographic studies of recombinant human betaine-homocysteine S-methyltransferase http://scripts.iucr.org/cgi-bin/paper?cnor=gr2115 'quickly transferred' Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus http://scripts.iucr.org/cgi-bin/paper?cnor=pu0115 5% increments from 5 to 30% for 3 min at each step Hope that helps, Sean
