Dear All, In every refinement round the wxc and wcu are out of control, although I modify the def file and change the values of wxc=1 and wxu=1 so they are tightly restrained, but it failed to fix the problem, wxc and wxu values keep fluctuating, in my latest refinement round wxc=256!! wxu=3. The Stereochemistry looks very loosely restrained and the gap between R-free and R-work is too big. I've been using phenix for almost 4 years and never had this problem, or once I set the values of wxc and wxu that will be it, never change. I also tried to fix wxc from the command line and didn't work either (phenix.refine refine.def fix_wxc=1.0). I will certainly appreciate any help. Thanks, Mohd
********************************** Mohd A. Salameh, Ph.D. Mayo Clinic Cancer Center Griffin Cancer Research building,Rm 331 4500 San Pablo Rd Jacksonville, FL 32224 Tel: (904) 953-0046 Fax: (904) 953-0277 salameh.m...@mayo.edu **********************************
