Hello, My aim is to calculate SIRAS/MIRAS phases using PHENIX. Which should be the correct column labels and associated "Differences" in the derivative data one should select to calculate SIRAS phases? I am supplying both native and derivative data and for the derivative data i am selecting F_HA, SigF_HA, DANO_HA, SigDANO_HA as labels and 'Anomalous' as differences. Should the differences not be 'Isomorphous+Anomalous' in case of SIRAS? If so, which column label is the correct one if i choose Iso+Ano as differences? Running SIRAS in SHARP requires Fs,DANOs, and ISYM for the derivative. It seems that columns F_HA(+),SigF_HA(+),F_HA(-), and SigF(-) can also be used for SIRAS as mentioned below, which confused me a little bit. http://www.ccp4.ac.uk/HAPPy/docshow.php?doc=design/input.html#input
Could someone please clarify? Thanks for your time and help. Yogesh
