Hi All, I have a question regarding developing inhibitor for UTP binding protein. Since UTP is a common nucleotide substrate for a lot of glycoenzymes similiar to ATP for kinases, developing potent inhibitor for UTP in vitro may not seem to be an impossible task, or at least it's technically feasible by using fragment-based approach, however, I was told that it is possible that UTP is so abundant in the cell that a potent inhibitor in vitro may not be able to work as an inhibitor in vivo. I was trying to search for evidence that a UTP inhibitor works in vivo but did not succeed. I wonder if any one can help to provide some examples that a UTP inhibitor works in vivo. This example would provide evidence for the druggability for UTP binding site for many glycoenzymes. Thanks a lot!
Eric
