Hello Vin,
have you tried fitting two glycerol molecules with complementary
occupancies?
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Fri, 8 Jan 2010, Vin Purp wrote:
Can a trained eye identify this blob? This is 1.65 Ang resolution. There are
very few components in the M.L. and protein purification buffers:
~1.5 ammonium sulfate, ~50 mM TRIS-HCl buffer pH 8.5, ~5 mM
2-mercaptoethanol, and ~25% glycerol. That's it.
Glycerol fits the 'forked' portion, but does not account for the opposite
end that looks like a carboxylate. Are there any common contaminants
associated with glycerol (from aging), or left overs from synthesis (dirty
batch)? Any help is appreciated.
V
