I was recently testing out the 30% PEGMME 5k, 0.1M NaOAc pH 4.5, 1M
NaCl method mentioned in a Hampton Research catalog and attributed to
Enrico Stura. I see that he has also just commented on this thread.
I found that at 80mg/ml, batch with 1.5ul:1.5ul protein to precipitant
ratio, lysozyme crystallized in about 1 hour. Jumping that up to
150mg/ml allowed for crystallization in minutes. Hanging drop behaved
similarly. I was using lysozyme from Sigma.
Jeff
Jeffrey Wilson, Ph.D.
University of Cincinnati College of Medicine
Molecular Genetics Department
231 Albert Sabin Way
MSB 3109A
Cincinnati, OH 45267-0524
(513) 558-1360
On Dec 21, 2009, at 11:12 AM, MARTYN SYMMONS wrote:
Dear All
checking out the Lysozyme crystallization methods on the web I
liked the Rigaku Instructions that I found:
(http://www.rigaku.com/protein/crystallization.html)
"...create a drop of 3ul lysozyme solution, and 3 ul of well
solution, respectfully, for a total drop size of 6ul..."
So perhaps sometimes I am just not respectful enough to deserve
crystals ?
good wishes to all
regards,
Martyn
-----------------------
Martyn Symmons
MRC-MBU Cambridge UK
'Chan fhiosrach mur feòraich.'
Gaelic proverb -
Nothing asked, nothing learned.
