Hello,

I would be interested in people experiences and opinions about what types of fluorescence microscope setups have worked adequately to distinguish small protein crystals from background/ autofluorescence etc. ...general comments? (regular dichroic mirros etc dont allow Trp peak to pass through for instance etc and quartz becomes qute expensive i think?) i am looking for the cheapest but effective system (ie i want to see 10 um size things)

regular sterefluor. microscope with filters + fiber UV light from the side? anybody has found something like that to work?

thank you very much for comments,
Tommi



Tommi Kajander, Ph.D., Docent
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. Box 65 (Street: Viikinkaari 1, 4th floor)
University of Helsinki
FIN-00014 Helsinki, Finland
Tel. +358-9-191 58903
Fax  +358-9-191 59940

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