Hello,
I would be interested in people experiences and opinions about what
types of fluorescence microscope setups have worked
adequately to distinguish small protein crystals from background/
autofluorescence etc. ...general comments?
(regular dichroic mirros etc dont allow Trp peak to pass through for
instance etc and quartz becomes qute expensive i think?)
i am looking for the cheapest but effective system (ie i want to see
10 um size things)
regular sterefluor. microscope with filters + fiber UV light from the
side? anybody has found something like that to work?
thank you very much for comments,
Tommi
Tommi Kajander, Ph.D., Docent
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. Box 65 (Street: Viikinkaari 1, 4th floor)
University of Helsinki
FIN-00014 Helsinki, Finland
Tel. +358-9-191 58903
Fax +358-9-191 59940