Dear All, Sorry for the non-ccp4 query. I have solved a crystal structure of an enzyme and woring on its biochemical aspect. We have a mutant of this enzyme and we are comparing some thermodynamic parameters of this enzyme with mutant( lke delH and delS, delG). we have done the expt at different temp. and know the km value at these temp. Now the question is how to get the value of delH and delS. The relation of these parameters is:- -RTlnK = delH - TdelS
Is the K in this relation is km or kd. If it can be treated as kd, so it should be 1/km. All suggestions are welcome. Thanks James
