We are trying to crystallize a small fragment (approximately 120aa) of a membrane protein. We encounter many problems in solubilizing the protein and would like to know if any one has experience in crystallizing proteins in the presence of TFE (Tetrafluoroethylene) (I understand that high concentration might have a problems with secondary structure), HFIP (Hexafluoro-2-propanol), TFA (Trifluoroacetic acid) etc. Appreciate any feedback, suggestion
