Hi James,
have a look at the program CROSSEC:
http://www.ccp4.ac.uk/html/crossec.html
should be straightforward to use. However you are at an advantage by
actually measuring the peak, as a) the beamline could be misaligned
and b) the chemical environment of your crystal might influence your
actual peak. In general it's saver to "miss" the peak on purpose (to
higher energy) in such a way that you still get some anomalous
difference for your peak wavelength if you are uncertain where to
collect.
Jürgen
On Oct 31, 2009, at 10:36 AM, james09 pruza wrote:
Dear All,
Please suggest the Peak wavelength of SeMet and the corresponding
f_prime and f_doubleprime.
In one of the journal I got it is 0.97905 A and Phenix manual shows
f_prime and f_doubleprime to be -3 and 4 respectively.
How can one deduce f_prime and f_doubleprime for any HA at a
particular wavelength.
Thanks.
James.
-
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-3655
http://web.mac.com/bosch_lab/
