Hi Radovan,
you can look at
"A Lipidic-Sponge Phase Screen for Membrane Protein Crystallization"
Wöhri, A. B., Johansson, L. C., Wadsten-Hindrichsen, P., Wahlgren, W.
Y., Fischer, G., Horsefield, R., Katona, G., Nyblom, M., öberg, F.,
Young, G., Cogdell, R. J., Fraser, N. J., Engström, S. & Neutze, R.
(2008). Structure 16, 1003-1009.
Valerie
Valérie Biou valerie.b...@lebs.cnrs-gif.fr
Laboratoire d'Enzymologie
et Biochimie Structurales
CNRS, avenue de la Terrasse tel (33/0)1 69 82 34 81
91198 Gif sur Yvette fax (33/0)1 6982 31 29
Le 30 Oct 2009 à 11:06, Radovan Spurny a écrit :
I would like to ask for recommendations and tips how to use lipids
for crystallization of membrane proteins. Especially I am interested
about the solubilization of lipids (E.coli lipids, POPC, POPE, POPG)
and how to mix them with protein.
Thanks a lot.
________________________________________
Radovan Spurny, PhD
Laboratory of Structural Neurobiology
Division of Pharmacology
Campus Gasthuisberg, ON1
Herestraat 49, PB 601
B-3000 Leuven
Belgium
e-mail: radovan.spu...@med.kuleuven.be
web: www.xtal.be
