To measure detergent concentrations in protein samples, the way that I recommend is ATR-FTIR, it is very accurate, fast (10min ) and requires as low as 10uL of protein sample.
Here is the reference:
ยท PVeesler, D. et al. Production and biophysical characterization of the CorA transporter from M. mazei. Analytical Biochem. (2009). 388 :115-121.
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