Hi marija, you could try mass-spec. It shouldn't be too hard if your protein is a soluble protein (membrane proteins are more complicated). As a side note, we have never checked Semet incorporation and I'm sure this is true for many others. If you use the common protocols (either use an auxotroph or do met biosynthesis in a wildtype strain) there's no reason why you wouldn't get good incorporation.
Bert Bert van den Berg University of Massachusetts Medical School Program in Molecular Medicine Biotech II, 373 Plantation Street, Suite 115 Worcester MA 01605 Phone: 508 856 1201 (office); 508 856 1211 (lab) e-mail: bert.vandenb...@umassmed.edu http://www.umassmed.edu/pmm/faculty/vandenberg.cfm -----Original Message----- From: CCP4 bulletin board on behalf of Marija Backovic Sent: Mon 9/28/2009 7:32 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] amino acid analysis for SeMet incorporation Dear all - Does anyone know of a facility that provides amino acid analysis for determination of SeMet incorporation in a recombinant protein? I contacted centers at Grenoble and Uppsala, but SeMet determination is not something they routinely do. Any suggestions would be highly appreciated! Best, Marija Marija Backovic, Ph.D. Pasteur Institute Department of Virology 25 rue du Dr Roux 75015 Paris France +33 (0)1 45 68 82 87
