Hi Salameh,
Not sure if you mean a graph of B-factor vs sequence, or a 3D structure plot.

Either way you can get such a plot using aline http://crystal.bcs.uwa.edu.au/px/charlie/software/aline/ which can produce a sequence alignment (or single sequence) coloured by B-factor (CA, Main, Side or All) and also output a pymol script to use those colours in pymol on the structure.

Cheers,
Charlie


Salameh, Mohd A., Ph.D. wrote:
Dear All,

I would like to prepare a plot showing the b-factor of only the alpha carbon and/or the backbone atoms and I wonder if somebody knows a module or software that I can use, I tried the structure analysis/Temperature factor analysis module in CCP4 but it plots the average b-factor of all atoms which I’m not interested in. appreciate your help. Thanks, Mohd

****************************************************

*****Mohd A. Salameh, Ph.D.*

Mayo Clinic Cancer Center

Griffin Cancer Research Building

4500 San Pablo Road

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Tel:(904) 953-0046

Fax:(904) 953-0277

*****salameh.m...@mayo.edu*

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--
Charlie Bond
Professorial Fellow
University of Western Australia
School of Biomedical, Biomolecular and Chemical Sciences
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