Some proteins can be purified at room temperature. Others would be degraded or may lose 'competency' (however you wish to define the latter). As a rule of thumb, *most* proteins will not be hurt by exposure to +4C, but e.g. detrimental protease activity should follow the normal kinetic rules and slow down considerably at +4C as opposed to +21C. There are many options out there and it's hard to anticipate all eventualities - a cold-capable purification system is not a luxury* but an essential tool in any protein lab.
Artem * this is a very clumsy reference to the immortal words of Ostap Bender from the book 'The Little Golden Calf'. People familiar with early Soviet literature will get the reference. -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Ho Leung Ng Sent: Monday, August 31, 2009 11:31 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Cold Cabinet Vs Fridge, FPLC I know several labs that keep their FPLCs at room temperature. Maybe the cold cabinet isn't necessary? ho
