Hello All,
I did diffraction with the exact same sample at 1 Å and at 1.54 Å and
noticed that the higher-resolution data from the 1 Å source is more
intense relative to the 1.54 Å source after normalizing cumulative
intensity between the two data sets. Is this effect from air
absorption or something else? If this is a known phenomenon, is there
some way to calculate what the wavelength dependent correction might
be (as a function of resolution) from the experimental conditions or
is the only hope to empirically determine the scaling parameters? The
sample is protein and it is shot in air at about 20 °C.
Thanks in advance for any input.
James
