Hi, everybody I have a crystal structure as homodimer, from which residues in the dimer interface need to be identified. Using PISA on the EBI website, residues involved in forming hydrogen bonds, salt bridges, disulfide bonds and covalent bonds could be identified. But hydrophobic interaction could also play an essential role on the dimer stablization. So does anybody know: 1. how to find those hydrophobic residues (software or on-line-server) 2. how to confirm that whether they play a role in the dimerization, except for site-mutation.
Thanks a lot! Hunter
