I forgot the mention this: does anyone happen to have some pictures of DDM crystals? It would be very very helpful for me to take a look and get a sense how they look like. I appreciate your inputs and help.
Thanks, Joe On Mon, Aug 10, 2009 at 4:47 PM, Joe <gch...@gmail.com> wrote: > Hi all, > > > I am doing initial crystallization screening for a membrane protein > purified in DDM. The actual concentration of DDM in the protein solution is > >> 2 CMC after the protein concentration step (Amicon MWCO 50 kD used). I > just found over 50 conditions out of 1000 give me crystals. Having worked > on soluble proteins, frankly they all look ugly to me to different degrees. > I have selected a few conditions to go after, hoping that they will grow big > enough for me to verify the crystal identity by gels. I wonder if there is > a quick way to tell if they are detergent crystals. I know a microscope > with UV capacity would do the job, but we probably will not get one very > soon. I have read through all threads on CCP4BBS regarding detergent > crystals. I have an impression that it is actually not easy to crystallize > detergents--please correct me if I am wrong. Your thoughts and comments are > helpful. > > Thank you for your attention. > > -- > Best regards, > > Joe > -- Best regards, Joe
