[ccp4bb] Crystallization of a protein/DNA complex--need your advice

[Joe]

Hi,

I try to crystallize a protein/DNA complex.  Is there a general 
guideline about size / affinity / symmetry of the DNA site?  My 
understanding is that the shorter DNA size preserving strong binding 
affinity is the better.  Is it right?  The protein is a homodimer.  I 
have options of using the native asymmetric DNA binding site or a 
symmetric site derived from sequence concensus.  I will try both  but I 
would like to hear your expert comments also.  Thank you in advance.

Best,

Joe