On a different note,it seems as if SeMet is not a regular experiment
in your lab. So I would recommend not to burn your crystals and rather
sacrifice the resolution versus loosing your SeMet anomalous signal
after couple of degrees into the data collection. It would also be
wise to run Mosflm STRATEGY ANOMALOUS to figure out where you should
start collecting your data.
Good luck,
Jürgen
On Jul 16, 2009, at 1:15 PM, Jerry McCully wrote:
Dear All:
Next week we are going to try some seleno-Met labeled crystals.
We checked the literature to try to find out the peak
wavelength that has been used for SAD or MAD data collection. But
they are slightly different ( may be 50 ev) in different papers.
I guess this is due to the discrepancy between the fluorescence
scanning and the theoretical vaules of f' and f''.
When we collect the data, which wavelength should we use?
Should we trust the scanning results?
Thanks a lot for your comments.
All the best,
Jerry McCully
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Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-3655
http://web.me.com/bosch_lab/
