Okay, it seems that the consensus is phage infection. Is there anything to
seal the diagnosis? Also, does anybody have literature on de-phaging
glassware? I am assuming that regular autoclaving will not do the trick?
Jacob
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
*******************************************
----- Original Message -----
From: "Jacob Keller" <j-kell...@md.northwestern.edu>
To: <CCP4BB@JISCMAIL.AC.UK>
Sent: Thursday, July 02, 2009 11:42 AM
Subject: [ccp4bb] Shredded E coli pellets
Dear crystallographers,
I recently expressed some new constructs, and found after my usual
expression protocol that the cell pellets were not compacted at the bottom
corner of the bottles us usual, but were instead smeared as a film on the
side, and further, were somewhat clumpy, like clots, and with a smaller
pellet in the usual location. The centrifugation was exactly as usual. I
noticed that there was also a bit more foam in the medium than usual, but
I am not convinced that this was the issue, although it might be a
symptom. My suspicion is that the constructs are lethal and cause cell
lysis, but I am not sure. Has anybody seen this phenomenon before, and
gotten to the bottom of it?
Jacob Keller
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
*******************************************
