You may also apply salting-in: if the reservoir concentration is lower
than that of the protein buffer, water may evaporate from the reservoir
into the drop, lowering the protein's solubility and thus maybe grow
crystals.
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Mon, 29 Jun 2009, rui wrote:
Dear All,
I have a peri domain protein that is stable in high salt concentration(500 mM),
if I dialysis to a
lower salt buffer and then concentrate, it'll preticipate out. If I need to
crystallize it, can I use
the high salt buffer? Is there any optimization kits that could help to
increase the solubility?
Thanks.
