Dear members, I am trying to crystallize a complex of two proteins prepared in Tris, NaCl and DTT. The problem is one of the proteins degrades over time and allows only the second protein to crystallize. While addition of protease inhibitors such as EDTA is one of the ways to go, I would like to know if there are other ways to fix this problem. Thank you Kumar
- [ccp4bb] Complex degradation Office 2004 Test Drive User
- Re: [ccp4bb] Complex degradation Herman . Schreuder