Re: [ccp4bb] Phasing at Low Resolution

[Roger Rowlett]

Regarding question 1, see: Acta Cryst. (2008). D64, 354-367  Towards a rational approach for heavy-atom derivative screening in protein crystallography J. Agniswamy, M. G. Joyce, C. H. Hammer and P. D. Sun Cheers. riya doreen wrote: I have two questions regarding derivatization:   (i) Is there a pH dependence to making derivatives ? i.e. effectiveness of getting derivatives at low vs high pH ?   (ii) Any thoughts on the effectiveness of Barium for phasing low resolution structures (especially for magnesium binding proteins ) ???   Thanks   -- Roger S. Rowlett Professor Colgate University Presidential Scholar Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@mail.colgate.edu