Hello Artem,
We express almost all our proteins in BL21 derivatives. It sounds
like you've worked with many proteins that express/behave better in
XL1-Blue?
ho
UC Berkeley
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XL1-Blue is a strain of E. coli. Whether it is or isn't an expression host
depends on the definition, and I am not going to argue semantics.
The T5 promoter works with regular garden variety RNApol of E. coli.
Therefore ANY E. coli strain is an 'expression host' for vectors that
contain this promoter.
I've expressed many proteins in XL1-Blue and I see no reason why you can't
express yours, either.
Artem
