Dear Li,
the samples should be devoid of aggregates, at least for the low
concentration samples. Some aggregation can be tolerated at the higher
concentrations. You should in principle measure samples with different
concentrations, such as 1, 2, 5,10 mg/mL.
The high concentration measurements will allow you to get a better
signal/noise ratio at the higher s values.
Cheers
Isabelle
**********Dr. Isabelle Billas, PhD.
Department of Structural Biology and Genomics
Institute of Genetics and of Molecular and Cellular Biology
IGBMC/ CNRS(UMR 7104) / INSERM (U964)/UDS/Collège de France
CEBGS
1, rue Laurent Fries
BP 10142
67404 ILLKIRCH CEDEX
FRANCE
Li Sheng a écrit :
Dear all,
Is there anyone with experience in small angle x-ray scattering? Could
anyone please tell me the requirements for protein sample purity and
concentration?
Thanx in advance.
Sincerely,
Li
__________________________________________
Email:s...@sibs.ac.cn
Institute of Biochemistry and Cell Biology
Institutes for Biological Sciences
Chinese Academy of Sciences
320 Yue-Yang Road, Shanghai 200031, China
Tel: +86-21-5492-1217
__________________________________________
